Dehydroabietic Acid Attenuates Atherosclerosis in Apolipoprotein E-Deficient Mice and VCAM-1 Expressions in-vitro

Dehydroabietic acid (DHA) is an analog of abietic (AA) for cardiovascular disease prevention, known to act pharmacologically against aging, inflammation, bacterial infections and cancer. The current research investigated the molecular mechanisms DHA on adhesiveness endothelial leukocytes activation NF-κB in TNF-α treated HAEC ApoE−/− mice experimental atherosclerosis. HAECs were tested toxicity using MTT assay at various concentrations. cell-surface expression CAM leukocyte adhesion molecule-1, ICAM -1, or human VCAM-1 was determined by ELISA test, followed western blot analysis. Endothelial cell-leukocyte involving U937 cells carried out determination p65 expression. fed with a high cholesterol diet every day oral administration (10 20 mg/kg/day). (5 10 mM) substantially reduced (p < 0.05) (U937) cell lines binding activated aortic cells. assays 32P-labelled NF-kB as probe demonstrated that pre-treatment decreased shifted bands density following stimulation TNF-α. Nuclear extracts immune assessment fluorescence staining showed significant decrease concentration nuclei Together these findings indicate inhibits nuclear trans localization TNF-α-induced p65, thus considerably suppresses expression, which contributes lower adherence suggesting helps preventing inflammatory atherosclerosis vivo.